AICAR

AICAR

Metabolic Signaling & Energy Regulation Research Compound

Product Overview

AICAR (5-Aminoimidazole-4-carboxamide ribonucleotide) is a specialized small-molecule research reagent used to interrogate cellular energy-sensing and metabolic signaling networks. In laboratory systems, AICAR serves as a chemically defined tool to activate AMP-activated protein kinase (AMPK), allowing researchers to profile downstream changes in substrate utilization, stress-response signaling, and transcriptional programs in both cell culture and animal models.

Key Research Applications

• Metabolic Flux & AMPK Profiling: Used in experimental workflows to map the phosphorylation-state and activation of AMPK-associated pathways.

• Glucose Transport Research: Targeted in studies evaluating insulin-stimulated uptake and GLUT-4 trafficking within muscle tissue models.

• Inflammatory Pathway Modulation: Utilized to quantify the impact of AMPK activation on NF-κB transcriptional programs and cytokine outputs in immune-signaling studies.

• Cell-Cycle & Apoptosis Analysis: Frequently employed to measure interactions with p38 MAPK signaling and p21-related checkpoints in survival assays.

• Vascular & Reproductive Biology: Evaluated for its role in regulating vascular smooth muscle proliferation and spermatozoa energy metabolism.

Why Researchers Choose AICAR

• Targeted Enzyme Activation: Acts as a potent analog to AMP, providing a high-fidelity subject for investigating peptide-independent AMPK activation.

• High Analytical Purity: Synthesized to a verified 99% HPLC purity, ensuring maximum accuracy and reproducibility in sensitive laboratory assays.

• Stability-Focused Format: Arrives in a lyophilized (powder) form to maintain structural integrity and maximize shelf life during laboratory handling.

• Broad Investigative Scope: Demonstrates multifaceted interactions across various metabolic, inflammatory, and reproductive physiological paradigms.

Technical Specifications

• Molecular Formula: C9_H15_N4_O8_P

• Molecular Weight: 338.213 g/mol

• CAS Number: 3031-94-5

• Purity: 99% HPLC

• Research Notice: AICAR is strictly for laboratory and scientific research use only. It is not approved for human or animal consumption, medical treatment, or therapeutic use.

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Validated Synthesis & Expedited Fulfillment

Clinical-Grade Synthesis & Purity

Our RUO compounds are engineered via precision synthesis, following meticulously validated protocols to ensure batch-to-batch consistency and uncompromising accuracy. To maintain verified purity, every production lot undergoes independent analytical testing—including HPLC and mass spectrometry—to confirm molecular identity and a purity level of 99%. For expedited research timelines, we provide same-day fulfillment from our facility for orders placed before 12 PM PST.

References:

• Corton JM et al., Eur J Biochem, 1995 229(2):558–565

• Hardie DG et al., Nat Rev Mol Cell Biol, 2012 13(4):251–262

• Winder WW et al., Am J Physiol, 2000 279(5):E1106–E1112

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Storage & Handling Guide

To ensure the long-term stability and reproducibility of your experimental results, please follow these standardized laboratory protocols for AICAR:

I. Long-Term Storage (Lyophilized Powder)

• Temperature: Store at -20°C for long-term stability. The lyophilized format is stable at room temperature for short-term transit but should be frozen upon arrival.

• Environment: Keep in a cool, dry place away from direct light. Ensure the vial remains tightly sealed to prevent moisture absorption (hygroscopy).

II. Reconstitution Protocol

• Solubility: AICAR is soluble in aqueous buffers or sterile water. Researchers should select solvent systems appropriate for nucleoside analogs and compatible with their specific in vitro or in vivo protocols.

III. Storage of Reconstituted Solution

• Short-Term: Once reconstituted, the solution should be stored at 2°C to 8°C and used within 7 days.

• Long-Term: For extended use, aliquot the solution into single-use volumes and store at -20°C or -80°C.

• Avoid Freeze-Thaw Cycles: Repeated freezing and thawing can degrade the compound’s structure. Always use fresh aliquots for each assay.

Research Use Only: This compound is intended for in vitro laboratory research and animal imaging studies. It is not for human or clinical use.